Project focuses on achieving the next major advance in medical imaging, enabling advanced analysis of cells or tissues
Berlin (Germany), 27 October 2017 – PicoQuant and the Humboldt University Berlin are co-hosting an Early Stage Researcher (ESR) within the framework of the Marie Skłodowska-Curie actions of the Innovative Training Networks H2020 BE-OPTICAL (http://beoptical.eu).
The ESR is working on a PhD thesis during his stay at PicoQuant that is entitled "Advanced Nanoscale Microscopy: Time-Resolved Super-Resolution Fluorescence Studies of Biological Structures".
The aim of the project lies in exploring novel strategies based on pulsed excitation coupled with nanosecond time-resolved detection to carry out multiplexed studies of complex dynamics in biological structures under optical super-resolution conditions. In the scope of this project, different pulsed interleaved excitation schemes will be combined with pattern-based fluorescence decay recognition to optimize the separation of multiple labels. Part of the project also includes the screening and full characterization of promising dye labels as well as identifying optimal sample preparation and experimental conditions, starting from artificial model systems up to studies in real cell. This latter part will be performed in collaboration with our partners.
A key aspect for this projects success is PicoQuant’s expertise in time-resolved and super-resolution microscopy via Stimulated Emission Depletion (STED).
This project has received funding from the European Union's Horizon 2020 Program for research, technological development and demonstration under grant agreement no. 675512.
PicoQuant is a leading research and development company specializing in optoelectronics, which was founded in 1996. The company, based in the science and technology park of Berlin-Adlershof, Germany, is a worldwide leader in the field of single photon counting applications. The product portfolio encompasses picosecond pulsed diode lasers and LEDs, photon counting instrumentation, fluorescence lifetime spectrometers, FLIM and FCS upgrade kits for laser scanning microscopes as well as time-resolved confocal and super-resolution microscopes. Since April 2008 Sales and Support in North America is handled by PicoQuant Photonics North America Inc. The PicoQuant group employs currently around 80 people.
Microscopy images demonstrating the resolution difference between confocal and STED imaging.